FastQCFastQC Report
Sun 20 Dec 2015
QC.1.trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameQC.1.trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977202
Sequences flagged as poor quality0
Sequence length50-107
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCA142891.4622360576421252No Hit
GGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAGT109841.124025534127028No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG87460.8950043082187716No Hit
CCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCAC84460.8643044119844209No Hit
GCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTC80810.8269528715659608No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG79430.8128309192981594No Hit
GTCCAGTCCTACAACCCCGAGAAGCAAGCTTCTCGGTTTGGGCTCTTCCC66180.6772397109297771No Hit
GTCGGTTTGCGGTACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGC63730.6521681290050573No Hit
GGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGA54610.558840444452631No Hit
GCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTC48040.491607671699403No Hit
CCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACT43780.44801381904662496No Hit
CCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTT43420.44432983149850286No Hit
CCCGCGTCTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTG43350.443613500586368No Hit
CCACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGCC42920.4392131821261111No Hit
CTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTC40870.41823491969930476No Hit
CCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTCA38390.39285633881224147No Hit
CCGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGG38370.39265167283734587No Hit
GTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTC38010.3889676852892237No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA36340.37187807638543513No Hit
GTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGAC35980.3681940888373131No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGAC35630.3646124342766388No Hit
CTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTTGT33830.3461924965360284No Hit
GGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGT33640.3442481697745195No Hit
CTCGCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTG31690.3242932372221915No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGA31290.3201999177242781No Hit
CGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGA28940.29615166567403667No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC28780.2945143378748713No Hit
GCCCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGC28010.28663469784138795No Hit
GCCCTGTTCAGACTCGCTTTCGCTACGGCTCCGACTTTTCATCTTAACCT27700.28346237523050505No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGAC25120.2570604644689634No Hit
GCCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGTGATC24690.2526601460087065No Hit
CCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTTG24260.24825982754844955No Hit
GTCATGGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGC24130.2469294987116277No Hit
CTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGC24010.24570150286225365No Hit
CCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTCA23870.24426884103798396No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG23810.24365484311329694No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT23310.23853819374090515No Hit
TGGGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAA23190.2373101978915311No Hit
CTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTC23130.23669619996684413No Hit
GGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTC22960.2349565391802309No Hit
CGCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTGAG22420.22943055785804778No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGA21390.2188902601509207No Hit
CCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTACTG20670.21152228505467655No Hit
CCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTAAG20600.21080595414254163No Hit
GCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAGTT19940.2040519769709845No Hit
CTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCT19900.20364264502119317No Hit
GTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGT19790.20251698215926697No Hit
CGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCGCTA19730.20190298423457992No Hit
CACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTC19620.20077732137265378No Hit
TCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCG19270.1971956668119795No Hit
CGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTCAA18810.19248834938937906No Hit
ATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCC18440.18870202885380913No Hit
GACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATC17910.18327838051907386No Hit
CTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT17680.1809247218077736No Hit
GCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTGAGA17250.17652440334751668No Hit
CTCGGTTTGGGCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGT17130.17529640749814265No Hit
CTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCTG17060.1745800765860078No Hit
CGCCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGTGAT16930.17324974774918595No Hit
AGGGACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTT16930.17324974774918595No Hit
CGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGGC16790.17181708592491624No Hit
CCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGACT16740.17130542098767707No Hit
GGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTT16680.17069142306299004No Hit
ACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCG16530.1691564282512725No Hit
GGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCG16510.16895176227637684No Hit
CGGGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAA16340.16721210148976362No Hit
CACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT15910.1628117830295067No Hit
GTCCTCTTCTGCACTCAAGTTTCCCAGTTTCCGATGCGCTTCCTCGGTTA15520.1588207965190411No Hit
GGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCC15480.15841146456924976No Hit
GGGACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTA14990.1533971481843058No Hit
CTCGCCTTAGATCCCGACTAACCCTGGGAGGACGAGCCTTCCCCAGGAAA14920.15268081727217095No Hit
CATGGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCC14570.1490991627114967No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGT14450.14787116686212268No Hit
CTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTAAGATC14440.14776883387467482No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT14220.14551750815082246No Hit
CGCTACTCATGCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACG13980.14306151645207438No Hit
CCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCAC13490.13804720006713045No Hit
GGGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAG13410.13722853616754777No Hit
CTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTG13360.13671687123030857No Hit
GCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTACT13190.13497721044369537No Hit
GCCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGATACTCTCCTC13050.13354454861942566No Hit
GTTTGGGCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGTTTTC12620.12914423015916873No Hit
CGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTG12580.1287348982093774No Hit
CACACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCT12570.12863256522192956No Hit
CCCTAGTTCAAACAGTGCTCTACCTCCATGATCCATCCTCCGAGGCTAAC12490.12781390132234688No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGATA12490.12781390132234688No Hit
ACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCG12230.12515324364870312No Hit
CACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGCCA12200.12484624468635963No Hit
GGTCATTTTGCCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGAT12080.1236182488369856No Hit
CCACTTAGCATAAATTTAGGGACCTTAACTGGTGATCTGGGCTGTTCCCC12070.12351591584953776No Hit
CCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGACT11910.1218785880503724No Hit
CTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTA11780.12054825921355053No Hit
GCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGGCT11740.12013892726375917No Hit
GTCCAGTCCTACAACCCCGAGAAGCAAGCTTCTCGGTTTGGGCTTTTCCC11640.11911559738928082No Hit
GGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGAC11430.11696660465287628No Hit
CACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGA11280.11543160984115873No Hit
GTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCGCTACGG11150.11410128100433688No Hit
CGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTCAA10850.1110312913809018No Hit
ACACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTT10830.11082662540600613No Hit
CGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACTC10790.11041729345621477No Hit
GCCTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATC10530.10775663578257105No Hit
CCCCGCGTCTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACT10400.10642630694574919No Hit
CTCCCCATCACAGCTTGTCCTTAAGACTGCAAGCATTTCACTCGCTGTCA10200.10437964719679246No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGC10010.10243532043528358No Hit
GGGGGCTTCACACTTAGATGCTTTCAGCGTTTATCCCTGCCGTTCATAGC9990.10223065446038793No Hit
TCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCT9930.1016166565357009No Hit
CTTTCTCTTCCTGCGGGTACTGAGATGTTTCAGTTCCCCGCGTCTGCCGC9930.1016166565357009No Hit
GGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCG9920.10151432354825307No Hit
CTCTCCTACCGCGCACCCTTACGGGTGCACCCACAGTTTCGGTATTATGC9900.10130965757335741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2750.073.3754355
TATCTCG2450.061.7701342
GTATGCC3200.061.480598
CGTATGC3250.060.534737
CGGGCCT2350.060.1054081
GTATCTC2550.059.3477751
TATGCCG3400.057.8640829
TCCAGTC15000.054.1449552
AGTCCTA15250.053.5881275
GCCCTCC1050.052.847781
GTCCAGT15450.052.5679171
CAGTCCT15850.051.559554
GTCCTAC16700.050.747686
TCCTACA16750.050.5961957
CCAGTCC16050.050.2884563
CCTACAA17100.049.5605968
GGCCTAT24900.048.8248671
CTACAAC17500.048.4277849
GGGGACC758.489042E-847.082571
GGGGACA551.8189894E-1145.97019698-99
Produced by FastQC (version 0.11.4)