FastQCFastQC Report
Wed 24 Jun 2015
SRR616268sub_trim2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616268sub_trim2_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length0-107
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCA174011.7401No Hit
CCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAGTTA133071.3307No Hit
CCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTCA128141.2814No Hit
TCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGG97030.9702999999999999No Hit
CTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGT95240.9523999999999999No Hit
TCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACT94180.9418No Hit
CCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCAC90580.9058No Hit
CCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTCA77780.7778No Hit
TCGGTTTGCGGTACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGCA74850.7485No Hit
TCCAGTCCTACAACCCCGAGAAGCAAGCTTCTCGGTTTGGGCTCTTCCCA69770.6977No Hit
TAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCA64720.6472No Hit
CCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGACT56490.5649000000000001No Hit
CCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTT48010.48009999999999997No Hit
AGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACG47350.4735No Hit
CCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACT44480.4448No Hit
CCCGCGTCTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTG44060.4406No Hit
CCACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGCC43340.4334No Hit
TTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCG42190.42189999999999994No Hit
CCGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGG42160.42160000000000003No Hit
CTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTC41440.4144No Hit
CCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGACT40310.4031No Hit
GCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAGTT40020.40020000000000006No Hit
TTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCG39540.39540000000000003No Hit
CCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTACTG37300.373No Hit
GTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTC36940.3694No Hit
CCCTGTTCAGACTCGCTTTCGCTACGGCTCCGACTTTTCATCTTAACCTC36920.3692No Hit
GAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTC36590.3659No Hit
ACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCG36430.3643No Hit
CTCGCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTG36360.3636No Hit
CGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGA34330.3433No Hit
CTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTTGT34270.3427No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGA32070.32070000000000004No Hit
CCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGTGATCT29570.2957No Hit
TCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCG27740.2774No Hit
CACACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCT26620.2662No Hit
TCATGGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCC26590.26589999999999997No Hit
TTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTA26470.2647No Hit
GAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCAC26460.2646No Hit
CCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTTG26130.2613No Hit
TCATTTTGCCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGATAC24840.2484No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT24520.24520000000000003No Hit
GACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATC23950.2395No Hit
CTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTC23370.23370000000000002No Hit
CGCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTGAG22760.22759999999999997No Hit
CTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT22510.2251No Hit
CCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTAAG22180.2218No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGA22070.2207No Hit
CCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTGAGAT21980.2198No Hit
CTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGA21480.21480000000000002No Hit
TCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCT21420.21419999999999997No Hit
CGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCG21020.21020000000000003No Hit
GCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGG20890.2089No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTCAGTCTCGCAGTCAAGCTCCCTT20550.2055No Hit
CGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCGCTA20390.2039No Hit
CTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCT20050.20049999999999998No Hit
CACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTC19900.199No Hit
CGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTCAA19010.1901No Hit
ATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCC18960.1896No Hit
CTCTACCTCCATGATCCATCCTCCGAGGCTAACCCTAAAGCTATTTCGGA18820.1882No Hit
CCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGATACTCTCCTCG18460.1846No Hit
CGGTACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGCAGTGTGACA18130.1813No Hit
CGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGGC18080.1808No Hit
CCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGGCTT18020.1802No Hit
CTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCTG17950.1795No Hit
CTCGGTTTGGGCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGT17880.17880000000000001No Hit
TCCTCTTCTGCACTCAAGTTTCCCAGTTTCCGATGCGCTTCCTCGGTTAA17690.1769No Hit
CGCCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGTGAT17510.1751No Hit
TATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACTCTTT17510.1751No Hit
TCATCGACTACGCCTCTCGGCCTCGCCTTAGATCCCGACTAACCCTGGGA17320.1732No Hit
CGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTG17230.17229999999999998No Hit
TTTGGGCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGTTTTCT17070.1707No Hit
AGGGACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTT17040.1704No Hit
TACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGCAGTGTGACATCA16730.1673No Hit
ACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCG16690.1669No Hit
CAACGCCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGT16650.1665No Hit
CACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT16600.166No Hit
CGGGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAA16470.1647No Hit
CCTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCT16150.16149999999999998No Hit
CGGCGTTGCTCCATCAGACTTGCGTCCATTGTGGAAGATTCCCTACTGCT15600.156No Hit
CGCTTTCCTTAGCCTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTA15180.1518No Hit
CTCGCCTTAGATCCCGACTAACCCTGGGAGGACGAGCCTTCCCCAGGAAA15090.15089999999999998No Hit
CATGGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCC14900.149No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT14870.1487No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGT14760.1476No Hit
CTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTAAGATC14510.1451No Hit
CCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCTACGTA14430.1443No Hit
TACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTA14330.1433No Hit
CCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCAC14310.1431No Hit
CGCTACTCATGCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACG14300.14300000000000002No Hit
CTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTG13770.1377No Hit
CCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGC13710.1371No Hit
TTTGCGGTACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGCAGTGT13470.13470000000000001No Hit
CCACTTAGCATAAATTTAGGGACCTTAACTGGTGATCTGGGCTGTTCCCC13390.1339No Hit
CTCCCCATCACAGCTTGTCCTTAAGACTGCAAGCATTTCACTCGCTGTCA13350.1335No Hit
CCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCC12910.1291No Hit
TCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCGCTACGGC12780.1278No Hit
CCCTAGTTCAAACAGTGCTCTACCTCCATGATCCATCCTCCGAGGCTAAC12630.1263No Hit
GCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGTTTTCTTTCTC12600.126No Hit
CCGATCAACCTCTCAGTTCGGCTACGTATCATCGCCTTGGTGAGCCATTA12500.125No Hit
CACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGCCA12340.12340000000000001No Hit
CCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGCG12320.1232No Hit
CTCCGACTTTTCATCTTAACCTCGCAAGCAAACGTAACTCGCCGGTTCAT12320.1232No Hit
TCTTACTTCCATATAGGAATGGGAAATCTCATCTCGAGGGGGGCTTCACA12270.1227No Hit
TCCAGTCCTACAACCCCGAGAAGCAAGCTTCTCGGTTTGGGCTTTTCCCA12260.12260000000000001No Hit
TCTTTCCGTCCTGTCGCGGGTAACCCGCATCTTCACGGGTACTATAATTT12180.12179999999999999No Hit
CTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTA11870.11869999999999999No Hit
CACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGA11860.1186No Hit
CTCTCCTACCGCGCACCCTTACGGGTGCACCCACAGTTTCGGTATTATGC11700.117No Hit
CTCCATCAGACTTGCGTCCATTGTGGAAGATTCCCTACTGCTGCCTCCCG11530.1153No Hit
TACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTC11400.11399999999999999No Hit
CCCCGCGTCTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACT11240.1124No Hit
CGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTCAA11000.11No Hit
ACACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTT10950.1095No Hit
CGGGCTTTGACTAATTGTAGGCACACGGTTTCAGGAACTGTTTCACTCCC10940.1094No Hit
CGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACTC10840.1084No Hit
CTTTCTCTTCCTGCGGGTACTGAGATGTTTCAGTTCCCCGCGTCTGCCGC10830.1083No Hit
CTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTC10500.105No Hit
CCCACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGC10490.10490000000000001No Hit
CTCCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGA10430.1043No Hit
TTCCGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAG10370.1037No Hit
CTACTCATGCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGAT10290.10289999999999999No Hit
GGGCTTCACACTTAGATGCTTTCAGCGTTTATCCCTGCCGTTCATAGCTA10210.1021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCT2350.070.185711
GCTCGGA1700.062.761314100-101
CATTCTC35900.056.9640355
CTCACTT36100.056.9276249
AGTCCTA15700.056.8675734
CCGGCAT35800.056.821671
GCATTCT36250.056.551634
GAGTTAA1004.1836756E-1156.48518100-101
GTCCTAC16100.056.074325
GGCATTC37000.055.6749273
CGGCATT37600.054.1232222
CAGTCCT16500.054.1103553
CTCGGAG3950.053.67819100-101
CCAGTCC16550.053.64552
TCCAGTC16400.053.0283361
GATGAGT9600.051.922268100-101
TCCTACA17600.051.2952586
AGGTGGA4450.050.990574100-101
TGAGTAG1250.050.589336100-101
CCTATTC27300.049.9807321
Produced by FastQC (version 0.11.3)