FastQCFastQC Report
Wed 24 Jun 2015
SRR616268sub_trim2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616268sub_trim2_2.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length1-93
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTC145611.4561No Hit
GGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCAC104871.0487No Hit
GTGGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCC90920.9091999999999999No Hit
GTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAA64420.6442No Hit
GAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAA52980.5297999999999999No Hit
CCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGTTA45460.45459999999999995No Hit
CACCAGTTAAGGTCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTT40480.40480000000000005No Hit
CTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTCG40470.4047No Hit
CAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTAAGCGAAACGTC39550.3955No Hit
ACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAAC37470.3747No Hit
CCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGA36700.367No Hit
GCACCCCGGAAGGGGAGTGAAACAGTTCCTGAAACCGTGTGCCTACAATT36100.361No Hit
GATGGATCATGGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGT32120.3212No Hit
GGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGA31580.3158No Hit
CTCTCCGAAATAGCTTTAGGGTTAGCCTCGGAGGATGGATCATGGAGGTA31540.3154No Hit
GAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAA31530.3153No Hit
GTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAAATTTATGC30120.30119999999999997No Hit
AAGCGAAACGTCGAACCCTCCGTCCAGGATCCTGAGTACGGCGGAACACG29170.2917No Hit
ACATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGC27230.27230000000000004No Hit
GAACTAGGGGCCCATCAAGGGTTACTGAATTCAGATAAACTCCGAATACC27130.2713No Hit
CGAAGTGGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGA25560.2556No Hit
CTTACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGG25120.2512No Hit
GCACAGACAACTAGGATGTTGGCTCAGAAGCAGCCACCATTTAAAGAGTG24720.24719999999999998No Hit
CGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAA23640.23640000000000003No Hit
GAATTCAGATAAACTCCGAATACCATCGATCTTACTCCGGGAGTCAGACA23620.2362No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGAT23340.23340000000000002No Hit
CGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCC23160.2316No Hit
AGTCGAGTGGCACTGCGCCGAAAATATACCGGGGCTAAGCATAATACCGA22630.22629999999999997No Hit
ATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGACAACTAGGATGTTGGC22600.22599999999999998No Hit
GGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGAC22100.22100000000000003No Hit
ATAGTAGCGAAGTTCCTGATGTCACACTGCCAAGAAAAGCTTCTAGTGAG22040.22039999999999998No Hit
CAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATCA21880.2188No Hit
CGATTCCCATAGTAGCGGCGAGCGAAGTGGGAAGAGCCCAAACCGAGAAG21260.21259999999999998No Hit
GTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCT21110.2111No Hit
CCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCG20960.2096No Hit
GAAAAGCACCCCGGAAGGGGAGTGAAACAGTTCCTGAAACCGTGTGCCTA19910.19910000000000003No Hit
AGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATA19540.1954No Hit
GAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATCTC19450.19449999999999998No Hit
CCGAATGGGGGAACCCAGTACACATCAGTGTATTGCTTGTCAGTGAATAC18930.1893No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGG18810.18810000000000002No Hit
ATCTCAGTACCCGCAGGAAGAGAAAGAAAACTCGATTCCCATAGTAGCGG18670.1867No Hit
CTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGAC16660.1666No Hit
GATCCGGAGATTTCCGAATGGGGGAACCCAGTACACATCAGTGTATTGCT16460.1646No Hit
GAGTAGCGAAAGATCAGTGAGAATCTGATCCACCGTATGACTAAGGTTTC16380.1638No Hit
GAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTAAGCGAA15960.1596No Hit
ATAGCTTTAGGGTTAGCCTCGGAGGATGGATCATGGAGGTAGAGCACTGT15870.1587No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATG15820.1582No Hit
GGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGGGGA15120.1512No Hit
CTAATACCGATATGCTTCGGGGAGCTATAAGTAAGCTTTGATCCGGAGAT14690.1469No Hit
GGAGGCTAAGGAAAGCGCACGGCTGGAAATGTGCGCCCAAGCAGCAAGTC14660.1466No Hit
ATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCT14470.1447No Hit
GGATCATGGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGTTAC14390.1439No Hit
ATCAGTGAGAATCTGATCCACCGTATGACTAAGGTTTCCTGGGGAAGGCT14320.1432No Hit
CCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGACAACTA14150.14150000000000001No Hit
GTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAAA14100.14100000000000001No Hit
CCTGATGTCACACTGCCAAGAAAAGCTTCTAGTGAGAAATAAACTACCCG14080.1408No Hit
AGAGCACTGTTTGAACTAGGGGCCCATCAAGGGTTACTGAATTCAGATAA13970.1397No Hit
CTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGGGGAGCTA13830.13829999999999998No Hit
CCGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAA13530.1353No Hit
GGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGTTACTGAATTC13480.1348No Hit
AGGAGGCTAAGGAAAGCGCACGGCTGGAAATGTGCGCCCAAGCAGCAAGT13440.1344No Hit
GAAGTGGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGAC13320.1332No Hit
CAGATAAACTCCGAATACCATCGATCTTACTCCGGGAGTCAGACAGTGAG13160.1316No Hit
GACCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAA13120.1312No Hit
AGTTTATTTTGTTTAAGCGATGGAGGGACGCAGGAGGCTAAGGAAAGCGC13100.131No Hit
AGTCAAAGCCCGTTAATGGGTAATGGCGTGCCTTTTGTAGAATGAACCGG13060.13060000000000002No Hit
GGAAAAGGATGTGGCGTTGCACAGACAACTAGGATGTTGGCTCAGAAGCA13000.13No Hit
ACTGAATTCAGATAAACTCCGAATACCATCGATCTTACTCCGGGAGTCAG12960.1296No Hit
GACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTA12840.1284No Hit
AATACCGATATGCTTCGGGGAGCTATAAGTAAGCTTTGATCCGGAGATTT12590.12589999999999998No Hit
GTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTA12580.1258No Hit
GTCGAACCCTCCGTCCAGGATCCTGAGTACGGCGGAACACGTGAAATTCC12530.1253No Hit
CAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAGTTCT12480.1248No Hit
CTGTGATGGGGAGCGAAATTATAGTAGCGAAGTTCCTGATGTCACACTGC12430.1243No Hit
AGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGA11840.11839999999999999No Hit
CCGGAGATTTCCGAATGGGGGAACCCAGTACACATCAGTGTATTGCTTGT11840.11839999999999999No Hit
GTCGATGCAGGAACCGAGCATTACGATCCTTGTCTAGCATTACCAAGACG11740.11739999999999999No Hit
AAGGTCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGAC11420.1142No Hit
CTCAGTACCCGCAGGAAGAGAAAGAAAACTCGATTCCCATAGTAGCGGCG11390.1139No Hit
AGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAACAGT11340.1134No Hit
CCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCC11250.11249999999999999No Hit
GAAAGCCCTGTAAGCGAAACGTCGAACCCTCCGTCCAGGATCCTGAGTAC11230.11230000000000001No Hit
GGCGTGCCTTTTGTAGAATGAACCGGCGAGTTACGTTTGCTTGCGAGGTT11150.11150000000000002No Hit
CGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGCATGAGTAGCG11150.11150000000000002No Hit
CAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGA11070.11069999999999999No Hit
CCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACC11010.1101No Hit
CGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGC10900.109No Hit
CTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGT10870.1087No Hit
GCCGGCATGAGTAGCGAAAGATCAGTGAGAATCTGATCCACCGTATGACT10850.1085No Hit
GCGCCACAGAAGGTGAAAGCCCTGTAAGCGAAACGTCGAACCCTCCGTCC10740.10740000000000001No Hit
CTAGTGAGAAATAAACTACCCGTACCGCAAACCGACACAGGTAGTCGAGG10730.10729999999999999No Hit
GCAGCCACCATTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGGCACTG10630.10629999999999999No Hit
GTTAGCCTCGGAGGATGGATCATGGAGGTAGAGCACTGTTTGAACTAGGG10600.106No Hit
GTGGGTGCACCCGTAAGGGTGCGCGGTAGGAGAGCGTTCTAAGGGCGTTG10430.1043No Hit
GGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAA10400.104No Hit
CTCGATTCCCATAGTAGCGGCGAGCGAAGTGGGAAGAGCCCAAACCGAGA10320.10319999999999999No Hit
AGTAGCGAAGTTCCTGATGTCACACTGCCAAGAAAAGCTTCTAGTGAGAA10240.10239999999999999No Hit
ACCGAAACTGTGGGTGCACCCGTAAGGGTGCGCGGTAGGAGAGCGTTCTA10120.10120000000000001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG302.0645815E-457.6878557
CGGAGAG250.007264465651.9190649
GCCTTGG26950.048.6245541
GGCACTA28600.045.5350766
CCTTGGC29400.044.4254072
CACTAGG29400.044.00178
GTGGGTA22000.040.6929781
CTTGGCA32250.040.5156563
TGGGTAG23850.037.53652
TTGGCAC36100.036.6741334
CAGGATG4100.034.809831
GCACTAG38950.033.2130977
GGTAGCG27050.033.10924
GGTGGAT6050.032.8832131
GGGTAGC27250.032.8661963
GTGGATG6100.032.6136782
AAGGGGC1800.031.2350832
AGGAACC3550.029.2501759
CCTCCGC1150.028.61567584-85
TAGCGGT30200.028.3663776
Produced by FastQC (version 0.11.3)