FastQCFastQC Report
Wed 21 Feb 2018
Trimmomatic_on_DRR024501sub_2_fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic_on_DRR024501sub_2_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284239
Sequences flagged as poor quality0
Sequence length2-251
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1951.8164264E-6413.74072245
CCCATAT2450.001480054277.43427235-239
AGATCGG1506.997652E-873.886635240-244
GTTTGCA3750.00799177850.59039235-239
TATCCCT1350.00911298126.6806683
TCATCTA5559.841226E-425.928762240-244
TCCAACT5750.005668565619.186916235-239
TGACACG1409.219545E-414.183988175-179
AACATTA9250.002248118.7898374
Produced by FastQC (version 0.11.5)