FastQCFastQC Report
Tue 29 Dec 2015
QC.1.trimmed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQC.1.trimmed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297633
Sequences flagged as poor quality0
Sequence length51-251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2950.0120.978518
GTATCTC2900.0110.7951351
CGTATGC3350.0106.533317
CTCGTAT3050.0105.31085
TCGTATG3650.097.777156
TCTCGTA3350.095.912214
TATCTCG3600.092.557262
ATCTCGT3950.081.343273
TATGCCG4400.081.110599
GCCGTCT3150.024.17009710-14
TGAACTC4002.5058851E-522.710964245
TGCCGTC3850.021.01150510-14
GTCTTCT3850.019.77553415-19
CGTCTTC4000.019.62876310-14
GTCTGAA5300.019.588972245
ACGTCTG3950.018.397408240-244
CGTCTGA4250.017.709436240-244
GCACACG4350.017.297253235-239
CCGTCTT4650.016.88495610-14
CACGTCT3750.015.918138240-244
Produced by FastQC (version 0.11.4)