FastQCFastQC Report
Tue 23 Jun 2015
SRR616268sub_trim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616268sub_trim_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences998658
Sequences flagged as poor quality0
Sequence length15-107
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCA173951.7418375459867141No Hit
CCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAGTTA132991.3316871241205699No Hit
CCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTCA128041.2821206058530548No Hit
TCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGG96970.971003086141602No Hit
CTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGT95210.953379435202041No Hit
TCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACT94110.9423646533648156No Hit
CCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCAC90510.9063162764429864No Hit
CCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTCA77730.7783445383704932No Hit
TCGGTTTGCGGTACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGCA74800.7490051649313378No Hit
TCCAGTCCTACAACCCCGAGAAGCAAGCTTCTCGGTTTGGGCTCTTCCCA69740.6983371684801003No Hit
TAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCA64680.6476691720288628No Hit
CCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGACT56450.5652585770103479No Hit
CCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTT47970.4803446224833727No Hit
AGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACG47340.4740361565220526No Hit
CCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACT44460.4451974549845893No Hit
CCCGCGTCTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTG44040.44099181101037593No Hit
CCACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGCC43320.4337821356260101No Hit
TTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCG42170.4222666818870925No Hit
CCGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGG42150.42206641312641563No Hit
CTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTC41430.41485673774204984No Hit
CCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGACT40300.403541552763809No Hit
GCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAGTT40020.40073779011433347No Hit
TTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCG39530.3958312054777512No Hit
CCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTACTG37260.37310070114093113No Hit
GTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTC36920.36969613220942504No Hit
CCCTGTTCAGACTCGCTTTCGCTACGGCTCCGACTTTTCATCTTAACCTC36900.3694958634487482No Hit
GAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTC36590.36639169765825735No Hit
ACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCG36430.36478954757284276No Hit
CTCGCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTG36350.36398847253013544No Hit
CGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGA34330.34376132770177575No Hit
CTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTTGT34260.3430603870394069No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGA32040.32083055460427895No Hit
CCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGTGATCT29570.29609736266069064No Hit
TCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCG27730.2776726366784224No Hit
CACACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCT26590.2662573173198432No Hit
TCATGGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCC26560.265956914178828No Hit
TTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTA26470.2650557047557823No Hit
GAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCAC26460.26495557037544387No Hit
CCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTTG26130.2616511358242762No Hit
TCATTTTGCCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGATAC24830.24863366638028234No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT24500.24532923182911467No Hit
GACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATC23940.23972170653016348No Hit
CTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTC23350.23381377809019704No Hit
CGCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTGAG22740.22770558088955378No Hit
CTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT22510.22540249014177025No Hit
CCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTAAG22180.2220980555906026No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGA22070.22099657740688003No Hit
CCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTGAGAT21980.2200953679838343No Hit
CTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGA21470.2149885145865752No Hit
TCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCT21420.21448784268488313No Hit
CGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCG20990.2101820643303313No Hit
GCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGG20890.20918072052694717No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTCAGTCTCGCAGTCAAGCTCCCTT20550.20577615159544108No Hit
CGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCGCTA20390.20417400151002646No Hit
CTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCT20050.2007694325785204No Hit
CACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTC19900.19926741687344415No Hit
CGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTCAA19010.1903554570233253No Hit
ATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCC18950.18975465074129483No Hit
CTCTACCTCCATGATCCATCCTCCGAGGCTAACCCTAAAGCTATTTCGGA18820.18845290379689544No Hit
CCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGATACTCTCCTCG18440.1846477973440357No Hit
CGGTACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGCAGTGTGACA18130.18154363155354486No Hit
CGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGGC18070.18094282527151437No Hit
CCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGGCTT18010.18034201898948388No Hit
CTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCTG17950.1797412127074534No Hit
CTCGGTTTGGGCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGT17880.1790402720450845No Hit
TCCTCTTCTGCACTCAAGTTTCCCAGTTTCCGATGCGCTTCCTCGGTTAA17680.17703758443831621No Hit
CGCCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGTGAT17500.17523516559222477No Hit
TATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACTCTTT17500.17523516559222477No Hit
TCATCGACTACGCCTCTCGGCCTCGCCTTAGATCCCGACTAACCCTGGGA17320.1734327467461333No Hit
CGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTG17230.17253153732308757No Hit
TTTGGGCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGTTTTCT17060.17082925285733455No Hit
AGGGACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTT17030.17052884971631932No Hit
TACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGCAGTGTGACATCA16730.16752481830616686No Hit
ACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCG16680.1670241464044748No Hit
CAACGCCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGT16650.16672374326345957No Hit
CACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT16600.1662230713617675No Hit
CGGGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAA16470.16492132441736812No Hit
CCTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCT16140.16161688986620046No Hit
CGGCGTTGCTCCATCAGACTTGCGTCCATTGTGGAAGATTCCCTACTGCT15590.15610949894758766No Hit
CGCTTTCCTTAGCCTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTA15160.15180372059303585No Hit
CTCGCCTTAGATCCCGACTAACCCTGGGAGGACGAGCCTTCCCCAGGAAA15080.15100264555032855No Hit
CATGGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCC14870.14889982356322184No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT14870.14889982356322184No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGT14740.14759807661882246No Hit
CTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTAAGATC14510.14529498587103892No Hit
CCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCTACGTA14430.14449391082833163No Hit
CCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCAC14310.14329229826427065No Hit
TACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTA14310.14329229826427065No Hit
CGCTACTCATGCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACG14290.14309202950359382No Hit
CTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTG13770.1378850417259963No Hit
CCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGC13690.13708396668328898No Hit
TTTGCGGTACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGCAGTGT13470.13488101031584385No Hit
CCACTTAGCATAAATTTAGGGACCTTAACTGGTGATCTGGGCTGTTCCCC13390.13407993527313655No Hit
CTCCCCATCACAGCTTGTCCTTAAGACTGCAAGCATTTCACTCGCTGTCA13350.1336793977517829No Hit
CCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCC12900.12917335063655425No Hit
TCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCGCTACGGC12760.12777146931181646No Hit
CCCTAGTTCAAACAGTGCTCTACCTCCATGATCCATCCTCCGAGGCTAAC12630.12646972236741708No Hit
GCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGTTTTCTTTCTC12600.12616931922640182No Hit
CCGATCAACCTCTCAGTTCGGCTACGTATCATCGCCTTGGTGAGCCATTA12500.1251679754230177No Hit
CACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGCCA12330.12346569095726466No Hit
CCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGCG12320.12336555657692623No Hit
CTCCGACTTTTCATCTTAACCTCGCAAGCAAACGTAACTCGCCGGTTCAT12320.12336555657692623No Hit
TCTTACTTCCATATAGGAATGGGAAATCTCATCTCGAGGGGGGCTTCACA12260.12276475029489575No Hit
TCCAGTCCTACAACCCCGAGAAGCAAGCTTCTCGGTTTGGGCTTTTCCCA12240.12256448153421892No Hit
TCTTTCCGTCCTGTCGCGGGTAACCCGCATCTTCACGGGTACTATAATTT12180.12196367525218843No Hit
CTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTA11870.11885950946169759No Hit
CACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGA11860.11875937508135918No Hit
CTCTCCTACCGCGCACCCTTACGGGTGCACCCACAGTTTCGGTATTATGC11700.11715722499594455No Hit
CTCCATCAGACTTGCGTCCATTGTGGAAGATTCCCTACTGCTGCCTCCCG11520.1153548061498531No Hit
TACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTC11400.11415319358579214No Hit
CCCCGCGTCTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACT11230.11245090912003909No Hit
CGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTCAA11000.11014781837225557No Hit
ACACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTT10950.1096471464705635No Hit
CGGGCTTTGACTAATTGTAGGCACACGGTTTCAGGAACTGTTTCACTCCC10920.10934674332954825No Hit
CGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACTC10840.10854566828684094No Hit
CTTTCTCTTCCTGCGGGTACTGAGATGTTTCAGTTCCCCGCGTCTGCCGC10830.10844553390650252No Hit
CTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTC10490.10504096497499646No Hit
CCCACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGC10480.10494083059465803No Hit
CTCCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGA10430.10444015869296595No Hit
TTCCGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAG10370.10383935241093548No Hit
CTACTCATGCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGAT10290.10303827736822817No Hit
GGGCTTCACACTTAGATGCTTTCAGCGTTTATCCCTGCCGTTCATAGCTA10200.10213706794518244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAGT9050.058.822716100-101
CTCACTT36050.057.330389
GTATATA352.1805533E-457.053424
AGGTCCC559.556181E-657.05204100-101
CTCGGAG4250.056.93536100-101
CCGGGAG5100.056.935356100-101
GCATTCT36550.056.4095234
GGCATTC37100.055.707823
CATTCTC37100.055.573265
CCGGCAT37650.055.350011
TCGGTGG2400.054.912586100-101
CGGCATT37800.054.6761932
TGAGAGG351.4492578E-553.181377100-101
GGCCCTA150.0066772953.03936498-99
GTCCTAC14650.052.4774675
AGTCCTA14400.052.3484954
CCTATTC27950.052.030471
CCAGTCC14750.051.783232
CAGTCCT14700.051.6197663
ACAGACA3650.051.58129100-101
Produced by FastQC (version 0.11.3)