FastQCFastQC Report
Tue 23 Jun 2015
SRR616268sub_trim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616268sub_trim_2.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences999136
Sequences flagged as poor quality0
Sequence length14-93
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTC145551.4567586394644974No Hit
GGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCAC104851.0494066873778944No Hit
GTGGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCC90890.909685968676937No Hit
GTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAA64420.6447570701085737No Hit
GAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAA52970.5301580565608686No Hit
CCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGTTA45460.45499311405053966No Hit
CACCAGTTAAGGTCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTT40480.4051500496428915No Hit
CTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTCG40460.40494987669346316No Hit
CAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTAAGCGAAACGTC39540.395741921019761No Hit
ACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAAC37470.3750240207539314No Hit
CCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGA36700.36731736220094163No Hit
GCACCCCGGAAGGGGAGTGAAACAGTTCCTGAAACCGTGTGCCTACAATT36070.36101191429394996No Hit
GATGGATCATGGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGT32120.3214777567818595No Hit
GGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGA31580.31607308714729526No Hit
GAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAA31520.3154725682990103No Hit
CTCTCCGAAATAGCTTTAGGGTTAGCCTCGGAGGATGGATCATGGAGGTA31520.3154725682990103No Hit
GTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAAATTTATGC30110.30136037536431476No Hit
AAGCGAAACGTCGAACCCTCCGTCCAGGATCCTGAGTACGGCGGAACACG29160.2918521602664702No Hit
ACATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGC27210.2723352976972104No Hit
GAACTAGGGGCCCATCAAGGGTTACTGAATTCAGATAAACTCCGAATACC27110.27133443295006887No Hit
CGAAGTGGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGA25560.2558210293693752No Hit
CTTACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGG25110.25131713800723826No Hit
GCACAGACAACTAGGATGTTGGCTCAGAAGCAGCCACCATTTAAAGAGTG24720.24741376549338628No Hit
CGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAA23640.23660442622425779No Hit
GAATTCAGATAAACTCCGAATACCATCGATCTTACTCCGGGAGTCAGACA23610.2363041668001153No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGAT23330.23350174550811903No Hit
CGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCC23150.23170018896326428No Hit
AGTCGAGTGGCACTGCGCCGAAAATATACCGGGGCTAAGCATAATACCGA22630.2264956922781283No Hit
ATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGACAACTAGGATGTTGGC22600.22619543285398586No Hit
GGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGAC22100.2211911091182782No Hit
ATAGTAGCGAAGTTCCTGATGTCACACTGCCAAGAAAAGCTTCTAGTGAG22040.22059059026999328No Hit
CAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATCA21860.21878903372513853No Hit
CGATTCCCATAGTAGCGGCGAGCGAAGTGGGAAGAGCCCAAACCGAGAAG21260.21278384524228933No Hit
GTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCT21110.21128254812157704No Hit
CCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCG20960.20978125100086475No Hit
GAAAAGCACCCCGGAAGGGGAGTGAAACAGTTCCTGAAACCGTGTGCCTA19910.1992721711558787No Hit
AGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATA19540.19556897159145503No Hit
GAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATCTC19440.1945681068443135No Hit
CCGAATGGGGGAACCCAGTACACATCAGTGTATTGCTTGTCAGTGAATAC18930.18946369663389168No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGG18810.18826265893732186No Hit
ATCTCAGTACCCGCAGGAAGAGAAAGAAAACTCGATTCCCATAGTAGCGG18660.18676136181660954No Hit
CTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGAC16660.16674406687377893No Hit
GATCCGGAGATTTCCGAATGGGGGAACCCAGTACACATCAGTGTATTGCT16440.16454216443006758No Hit
GAGTAGCGAAAGATCAGTGAGAATCTGATCCACCGTATGACTAAGGTTTC16380.16394164558178265No Hit
GAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTAAGCGAA15960.15973801364378823No Hit
ATAGCTTTAGGGTTAGCCTCGGAGGATGGATCATGGAGGTAGAGCACTGT15870.15883723537136085No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATG15800.1581366300483618No Hit
GGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGGGGA15120.1513307497677994No Hit
CTAATACCGATATGCTTCGGGGAGCTATAAGTAAGCTTTGATCCGGAGAT14690.1470270313550908No Hit
GGAGGCTAAGGAAAGCGCACGGCTGGAAATGTGCGCCCAAGCAGCAAGTC14660.14672677193094835No Hit
ATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCT14470.14482512891137944No Hit
GGATCATGGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGTTAC14390.1440244371136662No Hit
ATCAGTGAGAATCTGATCCACCGTATGACTAAGGTTTCCTGGGGAAGGCT14320.14332383179066713No Hit
CCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGACAACTA14140.14152227524581237No Hit
GTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAAA14100.14112192934695578No Hit
CCTGATGTCACACTGCCAAGAAAAGCTTCTAGTGAGAAATAAACTACCCG14070.1408216699228133No Hit
AGAGCACTGTTTGAACTAGGGGCCCATCAAGGGTTACTGAATTCAGATAA13970.13982080517567178No Hit
CTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGGGGAGCTA13830.13841959452967365No Hit
CCGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAA13530.13541700028824905No Hit
GGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGTTACTGAATTC13480.1349165679146783No Hit
AGGAGGCTAAGGAAAGCGCACGGCTGGAAATGTGCGCCCAAGCAGCAAGT13430.1344161355411075No Hit
GAAGTGGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGAC13320.13331518431925182No Hit
CAGATAAACTCCGAATACCATCGATCTTACTCCGGGAGTCAGACAGTGAG13160.13171380072382538No Hit
GACCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAA13120.13131345482496878No Hit
AGTTTATTTTGTTTAAGCGATGGAGGGACGCAGGAGGCTAAGGAAAGCGC13100.13111328187554047No Hit
AGTCAAAGCCCGTTAATGGGTAATGGCGTGCCTTTTGTAGAATGAACCGG13060.13071293597668385No Hit
GGAAAAGGATGTGGCGTTGCACAGACAACTAGGATGTTGGCTCAGAAGCA13000.13011241712839894No Hit
ACTGAATTCAGATAAACTCCGAATACCATCGATCTTACTCCGGGAGTCAG12960.12971207122954234No Hit
GACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTA12830.12841094705825834No Hit
AATACCGATATGCTTCGGGGAGCTATAAGTAAGCTTTGATCCGGAGATTT12590.12600887166511868No Hit
GTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTA12580.1259087851904045No Hit
GTCGAACCCTCCGTCCAGGATCCTGAGTACGGCGGAACACGTGAAATTCC12530.12540835281683374No Hit
CAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAGTTCT12480.12490792044326299No Hit
CTGTGATGGGGAGCGAAATTATAGTAGCGAAGTTCCTGATGTCACACTGC12410.12420731512026392No Hit
AGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGA11840.11850238606155718No Hit
CCGGAGATTTCCGAATGGGGGAACCCAGTACACATCAGTGTATTGCTTGT11840.11850238606155718No Hit
GTCGATGCAGGAACCGAGCATTACGATCCTTGTCTAGCATTACCAAGACG11740.11750152131441566No Hit
AAGGTCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGAC11420.11429875412356276No Hit
CTCAGTACCCGCAGGAAGAGAAAGAAAACTCGATTCCCATAGTAGCGGCG11390.1139984946994203No Hit
AGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAACAGT11330.11339797585113538No Hit
CCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCC11250.11259728405342215No Hit
GAAAGCCCTGTAAGCGAAACGTCGAACCCTCCGTCCAGGATCCTGAGTAC11230.11239711110399386No Hit
GGCGTGCCTTTTGTAGAATGAACCGGCGAGTTACGTTTGCTTGCGAGGTT11150.11159641930628063No Hit
CGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGCATGAGTAGCG11150.11159641930628063No Hit
CAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGA11060.11069564103385325No Hit
CCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACC11010.11019520866028247No Hit
CGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGC10900.1090942574384268No Hit
CTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGT10870.10879399801428434No Hit
GCCGGCATGAGTAGCGAAAGATCAGTGAGAATCTGATCCACCGTATGACT10850.10859382506485603No Hit
GCGCCACAGAAGGTGAAAGCCCTGTAAGCGAAACGTCGAACCCTCCGTCC10730.10739278736828621No Hit
CTAGTGAGAAATAAACTACCCGTACCGCAAACCGACACAGGTAGTCGAGG10730.10739278736828621No Hit
GCAGCCACCATTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGGCACTG10620.10629183614643052No Hit
GTTAGCCTCGGAGGATGGATCATGGAGGTAGAGCACTGTTTGAACTAGGG10600.10609166319700222No Hit
GTGGGTGCACCCGTAAGGGTGCGCGGTAGGAGAGCGTTCTAAGGGCGTTG10430.10439019312686161No Hit
GGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAA10400.10408993370271914No Hit
CTCGATTCCCATAGTAGCGGCGAGCGAAGTGGGAAGAGCCCAAACCGAGA10310.10318915543029177No Hit
AGTAGCGAAGTTCCTGATGTCACACTGCCAAGAAAAGCTTCTAGTGAGAA10240.1024885501072927No Hit
ACCGAAACTGTGGGTGCACCCGTAAGGGTGCGCGGTAGGAGAGCGTTCTA10120.10128751241072287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGG25400.054.6772161
GGCACTA28500.048.587796
CCTTGGC28850.047.991132
CACTAGG29000.047.6008538
CTTGGCA31300.044.1030273
GTGGGTA19700.041.7275051
TTGGCAC36100.038.358784
TGGGTAG22100.037.0020982
GCACTAG38800.035.6894877
AAGGGGC1700.033.0865332
CAGGATG4600.032.91891
AGCCCCG802.0413503E-532.4502532
GGTGGAT5450.031.7540111
GGGTAGC26200.031.216383
ACGCTGG4750.030.9731669
CCTCCGC651.816261E-830.34206884-85
AGGAACC4150.030.23789
GGTAGCG27100.029.8603124
GTGGATG6000.029.5657862
GGTACTC600.006231363428.8497
Produced by FastQC (version 0.11.3)