FastQCFastQC Report
Fri 16 Aug 2019
Trimmomatic on SRR6322569_1 _R1 paired_.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic on SRR6322569_1 _R1 paired_.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464045
Sequences flagged as poor quality0
Sequence length1-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCA208060.844383929676609No Hit
GTTCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGG198670.8062758594100352No Hit
CCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGG155940.6328618186762012No Hit
CGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGG105600.4285636017199361No Hit
CTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTGC61420.24926492819733406No Hit
CGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACC57120.23181394820305637No Hit
GTGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCC53600.21752849481239184No Hit
CGTTGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCCA53450.21691973969631237No Hit
CTGAGTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCA47790.19394937998291428No Hit
CCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCAG47640.19334062486683481No Hit
ATGGTCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGC43760.1775941591975796No Hit
CTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCGT42970.17438804891956114No Hit
TCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCG42200.17126310599035327No Hit
TGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCC38530.15636889748360927No Hit
CCCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCT37130.1506871830668677No Hit
TCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGAC36050.1463041462310956No Hit
CACGCTTCGGTTATGCCTGCCAAACGCTTGGAACAGAACTTTTGGTCACC34940.14179935837210764No Hit
CCGTTGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCC31140.1263775620980948No Hit
GCCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAG30650.12438896205223525No Hit
GTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCACAAG30420.12345553754091342No Hit
CCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTG30020.12183219056470154No Hit
CCTGAGTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGC29650.12033059461170556No Hit
CTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGGC29140.11826082721703542No Hit
GGAAAGTCCATGCCCGCACAAGCTGCGATGCTTGTAGTGTTCGTGCTAAG29040.11785499047298244No Hit
AACGGTTGTTGTGGCGCTTAATAAAGCCGACCAAAAACCGATTGGCATCT28930.11740857005452417No Hit
CCGCGAAATTTTGATCCGTTGACAATCTGACACTCATCTTCATCACTTCA28880.11720565168249768No Hit
GAGTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCACA27750.11261969647469912No Hit
GCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGGA27650.11221385973064617No Hit
CAAAGGGGTGATTCCATTGGAATCATTTTTGGTGCTAACTGTGAAGTAAT27490.11156452094016141No Hit
GTCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCC26730.10848016168535882No Hit
CGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCC26590.10791199024368467No Hit
GTCCATGCCCGCACAAGCTGCGATGCTTGTAGTGTTCGTGCTAAGCCCAA26230.10645097796509399No Hit
ATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCACAAGCTG26050.10572047182579865No Hit
GCATCTGCAGGCTGAGGAAAGTCCATGCCCGCACAAGCTGCGATGCTTGT25660.10413770852399207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)