FastQCFastQC Report
Fri 16 Aug 2019
Trimmomatic on SRR6322569_2 _R2 paired_.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic on SRR6322569_2 _R2 paired_.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464045
Sequences flagged as poor quality0
Sequence length1-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCA194310.7885813773693258No Hit
GTTCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGG177650.7209689758101009No Hit
CCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGG141380.5737719887420887No Hit
CGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGG99500.40380756033270493No Hit
CTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTGC55280.2243465521124817No Hit
GTGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCC52670.2137542130926992No Hit
CGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACC52600.21347012737186213No Hit
CGTTGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCCA49390.20044276788776177No Hit
CCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCAG42780.1736169591058605No Hit
CTGAGTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCA40780.1655002242248011No Hit
ATGGTCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGC37810.1534468729264279No Hit
CTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCGT37270.15125535450854186No Hit
TGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCC35680.14480255027809963No Hit
TCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCG34820.14131235427924407No Hit
CCCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCT33800.13717281948990379No Hit
TCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGAC33460.13579297456012368No Hit
CACGCTTCGGTTATGCCTGCCAAACGCTTGGAACAGAACTTTTGGTCACC32790.13307386837496882No Hit
CTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGGC28510.1157040557295017No Hit
C28220.1145271291717481No Hit
CAAAGGGGTGATTCCATTGGAATCATTTTTGGTGCTAACTGTGAAGTAAT27790.11278203117232032No Hit
GCCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAG27630.11213269238183556No Hit
CCGTTGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCC27410.11123985154491901No Hit
CCGCGAAATTTTGATCCGTTGACAATCTGACACTCATCTTCATCACTTCA26910.10921066782465419No Hit
CCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTG25990.10547696977936684No Hit
CGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCC25640.10405654117518146No Hit
GCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGGA25590.10385362280315497No Hit
AACGGTTGTTGTGGCGCTTAATAAAGCCGACCAAAAACCGATTGGCATCT24680.10016050843227296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)