FastQCFastQC Report
Thu 15 Aug 2019
SRR6322569_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6322569_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570876
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCA234750.9131128844798426No Hit
GTTCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGG221360.8610294701105772No Hit
CCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGG172940.6726889978357572No Hit
CGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGG117660.457665013792964No Hit
CTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTGC69170.2690522607858178No Hit
CGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACC63750.24796995265427035No Hit
GTGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCC58610.22797676745202802No Hit
CGTTGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCCA56660.220391804194368No Hit
CCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCAG54070.21031741709829646No Hit
CTGAGTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCA52730.2051051859366224No Hit
CTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCGT48590.189001725481898No Hit
ATGGTCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGC48230.18760142457279153No Hit
TCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCG47790.18588994568388362No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC46610.1813000704818124TruSeq Adapter, Index 8 (100% over 50bp)
TGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCC43670.1698642797241096No Hit
CCCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCT41880.16290167242605244No Hit
TCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGAC39900.15520001742596687No Hit
CACGCTTCGGTTATGCCTGCCAAACGCTTGGAACAGAACTTTTGGTCACC37560.14609806151677482No Hit
GCCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAG33740.13123931298125618No Hit
CCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTG33590.13065585426912849No Hit
CCGTTGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCC33440.13007239555700081No Hit
GTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCACAAG33180.12906106712264614No Hit
CCTGAGTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGC32950.12816643043071702No Hit
CTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGGC32050.12466567815795082No Hit
CCGCGAAATTTTGATCCGTTGACAATCTGACACTCATCTTCATCACTTCA31690.12326537724884437No Hit
AACGGTTGTTGTGGCGCTTAATAAAGCCGACCAAAAACCGATTGGCATCT31560.12275971303166702No Hit
GGAAAGTCCATGCCCGCACAAGCTGCGATGCTTGTAGTGTTCGTGCTAAG31280.12167059010236199No Hit
GCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGGA30500.118636604799298No Hit
CGCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCC30100.11708071490029079No Hit
GAGTGATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCACA30090.11704181765281561No Hit
GTCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCC30040.11684733141543971No Hit
CAAAGGGGTGATTCCATTGGAATCATTTTTGGTGCTAACTGTGAAGTAAT29310.11400783234975159No Hit
GTCCATGCCCGCACAAGCTGCGATGCTTGTAGTGTTCGTGCTAAGCCCAA28820.1121018672234678No Hit
ATCGCGGTCTGCATCTGCAGGCTGAGGAAAGTCCATGCCCGCACAAGCTG28050.10910677916787896No Hit
GCATCTGCAGGCTGAGGAAAGTCCATGCCCGCACAAGCTGCGATGCTTGT27700.10774537550624766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)