FastQCFastQC Report
Thu 24 Mar 2016
DRR054113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR054113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163380
Sequences flagged as poor quality0
Sequence length116-28874
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGAG8300.097.195871
AAAGAGA10250.088.244941
GGAGAGA5750.080.778871
AGAGAGA18100.074.284141
GAGAGAG11250.060.8439642
GAGAGAT13300.056.9799463
GGGGAGA2201.11415175E-555.5596241
AGAGAGG4653.6379788E-1252.5725482
GAGAGAA6600.051.855652
AAAGGAG3303.9413862E-851.855651
AAAGGGA1954.113624E-450.1461223
AGGGAAG1500.00919723748.8924672
GGGAAGA2055.264359E-447.699971
GAGAGGA4803.1832315E-1045.8366933
AGAGAAT4803.1832315E-1045.8366933
AGAGATA9250.044.9282155
GGAGAAT2754.169266E-544.44771
AGAGATC5055.2204996E-1043.5675473
AAAAGGG2309.2795683E-442.515192
AGAAGAG5804.3655746E-1142.148681
Produced by FastQC (version 0.11.4)