FastQCFastQC Report
Wed 5 Jul 2017
Trimmomatic_on_DRR024501sub_1_fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic_on_DRR024501sub_1_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297724
Sequences flagged as poor quality0
Sequence length2-251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC150.0071347752.15664740-44
TCGGAAG2007.2759576E-1249.763603245
GGAAGAG2200.00208455345.23964245
AGATCGG2557.2759576E-1226.020185245
GATCGGA3055.456968E-1221.75458245
CGGAAGA2954.465346E-714.917212240-244
ATCGGAA4408.185452E-1114.547363240-244
GATGCAG5550.00684224266.170185205-209
Produced by FastQC (version 0.11.5)