FastQCFastQC Report
Fri 16 Aug 2019
Trimmomatic on SRR6322564_1 _R1 paired_.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic on SRR6322564_1 _R1 paired_.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669555
Sequences flagged as poor quality0
Sequence length1-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCA69700.41747651320261986No Hit
CCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGG63730.38171848187091767No Hit
CTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCGT63470.38016118067389215No Hit
GTTCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGG59090.3539266451240001No Hit
CTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTGC59020.35350737172480096No Hit
TCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCG35220.21095441599707707No Hit
CGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGG30130.18046725025530755No Hit
CGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACC24870.14896184911548288No Hit
CCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCAG24720.1480634061171989No Hit
CCCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCT21160.12674035895792593No Hit
TGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCCAGTA20320.12170907816753566No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC16800.10062561580780507TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)