FastQCFastQC Report
Thu 15 Aug 2019
SRR6322564_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6322564_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760461
Sequences flagged as poor quality0
Sequence length251
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC299421.7008045051835854TruSeq Adapter, Index 3 (100% over 50bp)
GCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCA79050.44903011199907295No Hit
CTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCGT72390.411199112050764No Hit
CCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGG70490.40040648443788307No Hit
CTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTGC66100.3754698343218055No Hit
GTTCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGG65640.3728568823734238No Hit
TCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCG41040.2331207564382284No Hit
CGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGG33970.19296082105766615No Hit
CCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCAG28020.1591628556383811No Hit
CGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACC27870.15831080608999576No Hit
CCCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCT23790.13513505837391457No Hit
TGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCCAGTA22120.12564890673522447No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATGC18650.10593816051591032TruSeq Adapter, Index 3 (98% over 50bp)
CCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTG18340.10417725811591395No Hit
TCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGAC18270.10377963499333413No Hit

[FAIL]Adapter Content

Adapter graph