FastQCFastQC Report
Fri 16 Aug 2019
Trimmomatic on SRR6322564_2 _R2 paired_.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic on SRR6322564_2 _R2 paired_.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669555
Sequences flagged as poor quality0
Sequence length1-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCA77780.46587264271018325No Hit
CCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGG55820.3343405877614095No Hit
CTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCGT55390.33176505116632876No Hit
CTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTGC51180.3065487510144919No Hit
GTTCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGG47250.28300954445945176No Hit
TCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCG31600.1892719916384905No Hit
CCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCAG27150.16261818268939926No Hit
TGACAATCTGACACTCATCTTCATCACTTCACTGGTATCGTCGCCCAGTA20010.1198522959710821No Hit
CGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACC18650.11170641278664074No Hit
CGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGG18410.11026890398938638No Hit
CCCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCT17880.10709440539544968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)