FastQCFastQC Report
Fri 16 Aug 2019
Trimmomatic on SRR6322567_2 _R2 paired_.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic on SRR6322567_2 _R2 paired_.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718252
Sequences flagged as poor quality0
Sequence length1-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCA88930.5175608700004424No Hit
GTTCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGG66000.3841112945016214No Hit
CAATGATATTGCTACTTTAGATGAAAAACAATGAAACAAGTCAAGGAAGA63650.3704346044701243No Hit
GCGGGCAGAACCGTTTTAACCGACGCACGGCATAGCTAACCGGTGGCGCA61120.3557103381808954No Hit
GCCCAATGATATTGCTACTTTAGATGAAAAACAATGAAACAAGTCAAGGA56030.32608720955948256No Hit
CTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCTGC48390.2816234172868706No Hit
CCTGCATGTAGATCCCTATAAGCCATGTTTTGTTCCAGAACCTGTTCAGG43960.25584140161047386No Hit
CTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCGT42350.24647141397187372No Hit
CGGGCAGAACCGTTTTAACCGACGCACGGCATAGCTAACCGGTGGCGCAT42350.24647141397187372No Hit
CGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACCGG36620.2131235697674148No Hit
TCTCGACATTGCTTTTCGCGCATGCGGTGCACCTCGGAGGCTCGGCTCCG28030.16313090280121892No Hit
CCGTTTTCTTCCCGGATCGTTGAAACCCGCTGCTAGCTATGTGGGCCCAG27680.16109394896674062No Hit
CGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGACC25670.1493960140887367No Hit
CGCCCAATGATATTGCTACTTTAGATGAAAAACAATGAAACAAGTCAAGG24350.14171378819870425No Hit
CCCAATGATATTGCTACTTTAGATGAAAAACAATGAAACAAGTCAAGGAA22410.130423244087596No Hit
CTTCTAACTCACACCAAAAAGAGGCGAGATCACATGGCATCATTGAGCAA21750.12658213114257977No Hit
CCCTGCTACATGCGGCTCTACACCCATAGCCGATTTATTTAAAATTCGCT19170.1115668714484255No Hit
C17510.10190589040489986No Hit
TCGCGCTTCCTTAACTATCAGTTGACCGAACAGTATCGAGACCTTGGGAC17310.10074191678519798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)