FastQCFastQC Report
Tue 18 Aug 2015
SRR616268sub_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616268sub_2.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length93
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTC92160.9216No Hit
TGTGGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTC68270.6827No Hit
GTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAA64420.6442No Hit
TGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTT53480.5348No Hit
TCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTAAGCGAAACGT37840.3784No Hit
GCACCCCGGAAGGGGAGTGAAACAGTTCCTGAAACCGTGTGCCTACAATT36100.361No Hit
TGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTA36040.3604No Hit
GATGGATCATGGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGT32120.3212No Hit
TGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCA31580.3158No Hit
TCACCAGTTAAGGTCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGT28890.2889No Hit
AAGCGAAACGTCGAACCCTCCGTCCAGGATCCTGAGTACGGCGGAACACG28430.2843No Hit
ATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGC28280.2828No Hit
ACATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGC27230.27230000000000004No Hit
TCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTC26190.26189999999999997No Hit
CGAAGTGGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGA25550.2555No Hit
CTTACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGG24950.24949999999999997No Hit
TGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAAATTTATG24360.24359999999999998No Hit
CGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAA23630.2363No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGAT23340.23340000000000002No Hit
CGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCC23160.2316No Hit
CTCTCCGAAATAGCTTTAGGGTTAGCCTCGGAGGATGGATCATGGAGGTA23140.2314No Hit
GGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGA22840.2284No Hit
GTGGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCC22160.22160000000000002No Hit
ACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAAC21360.21359999999999998No Hit
CCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCG20960.2096No Hit
TGAACTAGGGGCCCATCAAGGGTTACTGAATTCAGATAAACTCCGAATAC20680.20679999999999998No Hit
GAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAA19840.19840000000000002No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGG18800.188No Hit
GTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCT18800.188No Hit
ATCTCAGTACCCGCAGGAAGAGAAAGAAAACTCGATTCCCATAGTAGCGG18670.1867No Hit
TTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCC18260.18259999999999998No Hit
TGAATTCAGATAAACTCCGAATACCATCGATCTTACTCCGGGAGTCAGAC18080.1808No Hit
AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT17650.17650000000000002Illumina Single End PCR Primer 1 (100% over 50bp)
TCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTG17290.1729No Hit
GAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAA16940.1694No Hit
GGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCAC16770.16770000000000002No Hit
CTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGAC16660.1666No Hit
AGTCGAGTGGCACTGCGCCGAAAATATACCGGGGCTAAGCATAATACCGA15980.1598No Hit
GAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTAAGCGAA15960.1596No Hit
ATAGCTTTAGGGTTAGCCTCGGAGGATGGATCATGGAGGTAGAGCACTGT15870.1587No Hit
TTACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGA15540.1554No Hit
GGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGAC15260.1526No Hit
CTAATACCGATATGCTTCGGGGAGCTATAAGTAAGCTTTGATCCGGAGAT14690.1469No Hit
GGAGGCTAAGGAAAGCGCACGGCTGGAAATGTGCGCCCAAGCAGCAAGTC14660.1466No Hit
TCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATC14640.1464No Hit
ATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCT14470.1447No Hit
ATCAGTGAGAATCTGATCCACCGTATGACTAAGGTTTCCTGGGGAAGGCT14320.1432No Hit
GTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAAA14090.1409No Hit
CTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGGGGAGCTA13830.13829999999999998No Hit
AGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATA13600.136No Hit
TTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGT13580.1358No Hit
TGAGTAGCGAAAGATCAGTGAGAATCTGATCCACCGTATGACTAAGGTTT13570.1357No Hit
CCGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAA13530.1353No Hit
AGGAGGCTAAGGAAAGCGCACGGCTGGAAATGTGCGCCCAAGCAGCAAGT13440.1344No Hit
GAAGTGGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGAC13320.1332No Hit
GACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTA12840.1284No Hit
TCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGTT12780.1278No Hit
TGAAAAGCACCCCGGAAGGGGAGTGAAACAGTTCCTGAAACCGTGTGCCT12700.127No Hit
TGCACAGACAACTAGGATGTTGGCTCAGAAGCAGCCACCATTTAAAGAGT12690.12689999999999999No Hit
GTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTA12580.1258No Hit
GTCGAACCCTCCGTCCAGGATCCTGAGTACGGCGGAACACGTGAAATTCC12530.1253No Hit
TCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGACAACT12500.125No Hit
CTGTGATGGGGAGCGAAATTATAGTAGCGAAGTTCCTGATGTCACACTGC12430.1243No Hit
ATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGG12340.12340000000000001No Hit
GGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGTTACTGAATTC12220.1222No Hit
CAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAGTTCT12200.122No Hit
CTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTCG12140.12140000000000001No Hit
TATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGACAACTAGGATGTTGG11990.11989999999999999No Hit
TTCCGAATGGGGGAACCCAGTACACATCAGTGTATTGCTTGTCAGTGAAT11930.1193No Hit
TATAGTAGCGAAGTTCCTGATGTCACACTGCCAAGAAAAGCTTCTAGTGA11870.11869999999999999No Hit
AGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGA11840.11839999999999999No Hit
GTCGATGCAGGAACCGAGCATTACGATCCTTGTCTAGCATTACCAAGACG11740.11739999999999999No Hit
TGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGA11680.1168No Hit
CACCAGTTAAGGTCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTT11600.116No Hit
AGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAACAGT11340.1134No Hit
GACCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAA11120.1112No Hit
CAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGA11070.11069999999999999No Hit
CCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACC11010.1101No Hit
CGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGCATGAGTAGCG10980.10980000000000001No Hit
CGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGC10900.109No Hit
CTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGT10870.1087No Hit
GGAAAAGGATGTGGCGTTGCACAGACAACTAGGATGTTGGCTCAGAAGCA10850.1085No Hit
CGATTCCCATAGTAGCGGCGAGCGAAGTGGGAAGAGCCCAAACCGAGAAG10650.1065No Hit
GCAGCCACCATTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGGCACTG10630.10629999999999999No Hit
TCGATTCCCATAGTAGCGGCGAGCGAAGTGGGAAGAGCCCAAACCGAGAA10610.10610000000000001No Hit
GTTAGCCTCGGAGGATGGATCATGGAGGTAGAGCACTGTTTGAACTAGGG10600.106No Hit
TTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGT10590.10590000000000001No Hit
GGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAA10400.104No Hit
CTCGATTCCCATAGTAGCGGCGAGCGAAGTGGGAAGAGCCCAAACCGAGA10320.10319999999999999No Hit
TGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATCT10290.10289999999999999No Hit
CATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCG10080.1008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG2450.076.366489
GAGCGTC3000.062.365963
TCGTGTA3050.059.9169738
GTCGTGT3000.059.4652187
AGCGTCG3200.055.7486464
GCGTCGT4000.044.598915
TAGATCT2350.039.8000926-27
AAGAGCG4850.039.458221
GTAGATC2450.038.175624-25
GTGTAGA2400.038.0589122-23
AGTGTAG2400.038.0589122-23
TGTAGGG2700.034.6460210-11
CAGGATG4100.033.946321
GGTGGAT6050.033.0696071
GTGGATG6100.032.79692
AAGGGGC1800.031.4105632
GTGGGTA22000.030.2463932
GCCTTGG26950.030.0179331
CGTATCA3250.029.44779842-43
AGGAACC3550.029.415989
Produced by FastQC (version 0.11.3)